Journal: Cells

Article Title: Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients

doi: 10.3390/cells10113099

Figure Lengend Snippet: The process of reprogramming of Turner syndrome PBMCs into hiPSCs and characterization of hiPSCs-TS and colonies. ( A ) Schematic protocol used to reprogramming PBMCs into hiPSCs. ( B ) PBMCs with ten days of expansion in a specific medium for enrichment erythroblastic populations; the emergence of the first hiPSCs-TS colonies maintained in hESCs medium on MEF. ( C ) Characterization of hiPSCs-TS of Pt.1, Pt.2, Pt.3, Pt.4 and Pt.5. Phase contrast image showed hiPSCs maintained in mTeSR-1 medium on Geltrex; positively stain of alkaline phosphatase; Immunofluorescence assay showed the presence of pluripotency markers OCT4, SOX2, NANOG. Nuclei were stained with Hoechst (blue). Scale bars s: 200 μm and 100 μm. The data was compiled from at least three technical replicates. ( D ) Quantification of the relative expression of OCT4 , SOX2 , NANOG , KLF4 , and c-MYC genes in all hiPSCs lines and subclones (p15). Individual quantifications by qRT-PCR were normalized using the Β-ACTIN gene. The ESCs-H1 line was used as a positive control and was not considered in the statistical model. All qRT-PCR from hiPSCs-TS and control data were compiled from at least three biological and technical replicates. p values were calculated by Two-way ANOVA, followed by Tukey’s multiple comparisons test as appropriate (letters a-b-c show difference between groups). Erros bars denote represent SD.

Article Snippet: The human embryonic stem cell (hESCs) line H1 (cat.# WA01-cGMP Material) was bought from WiCell International Stem Cell Bank, and was first published by Thomson et al. [ ].

Techniques: Staining, Immunofluorescence, Expressing, Quantitative RT-PCR, Positive Control

Journal: Cells

Article Title: Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients

doi: 10.3390/cells10113099

Figure Lengend Snippet: Cytogenetic analysis of all lines of hiPSCs-TS and controls. ( A – C ) phase contrast and karyotype of hESCs-H1 (p30) and hiPSCs male and female control (p20). 2). ( D – G ) phase contrast and karyotype of hiPSCs TS (p20). 3). ( H ) Phase contrast and karyotype of hiPSCs TS–Pt.2 (p20). Cytogenetic analyses revealed reciprocal chromosome translocations between chromosomes 11 with breakage at the q arm, and chromosome 12 with a breakpoint at the p arm (der(11;12)(q10;10), der(11;12)(p10;p10)). Scale bars indicate 400 μm.

Article Snippet: The human embryonic stem cell (hESCs) line H1 (cat.# WA01-cGMP Material) was bought from WiCell International Stem Cell Bank, and was first published by Thomson et al. [ ].

Techniques: